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MicroRNA in situ Detection
Phylogeny’s is pleased to offer the only commercial in situ detection of miRNA. in situ miRNA hybridization methods offer sensitive detection of miRNAs in specific cell(s) or sub-cellular organelles. Combined with our comprehensive tissue procurement and other services, the in situ miRNA hybridization service makes exploring the role of miRNA in your experimental system easily accessible. In addition, the ability to detect miRNA in FFPE tissue, in situ miRNA hybridization opens the vast collections of archival formalin fixed paraffin embedded cell and tissue materials for scientific inquiry. < back to services
MicroRNAs (miRNA) are small (18-23 nucleotides), noncoding molecules that elicit sequence-specific gene silencing of target message. The miRNAs silence specific mRNAs by annealing to their untranslated regions (UTRs) and have been shown to regulate a variety of cellular processes such as embryogenesis, cell differentiation, and oncogenesis. To date, most studies of miRNA expression have used solution phase RT-PCR in real time mode or on microchip arrays. These methods are limited by their inability to identify specific populations of cells that express particular miRNAs. They are also unable to characterize differences in intracellular localization of miRNAs which is a key component of miRNA activity.
Detecting miRNAs in situ offers more precise characterization of miRNA related phenomena and can be approached using modifications of well established methods of in situ hybridization. Two methods can be used to detect miRNAs‐
- sensitive in situ detection of the primary transcript miRNA and precursor miRNA by RT in situ PCR, and
- detection of mature miRNA by in situ hybridization using LNA modified probes.
Applied together on serial tissue sections, they can be used to characterize the processing of a given miRNA, and to correlate its expression pattern with specific cell types or functions. By combining this data with co-localization and/or immunohistochemistry analyses on serial sections, much information about the modulation of miRNA expression and its’ putative targets can be determined.
To learn more about the details of the service go here
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